1980•HortScienceOpen access

Seedlings from Undeveloped Ovules of Ripe Fruits of Polyembryonic Citrus Cultivars1

A. Starrantino, Francesco Paolo Russo

Abstract

Abstract Embryos, pseudobulbils, and embryogenic callus were obtained by in vitro culture of undeveloped ovules excised from ripe fruits of the navel orange group ( C . sinensis [L.] Osbeck) and lemon (C. limon [L.] Burm. f.). Initial culturing was on Murashige & Skoog (MS) medium in 1% agar. Large numbers of seedlings were produced by subculturing the embryos, pseudobulbils, and embryogenic callus on fresh MS medium with added 6-methylaminopurine.

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Available abstract

Key concepts: Polyembryony, Ovule, Biology, Citrus × sinensis, Callus, Botany, Orange (colour), Agar