2013•Journal of Biotechnology Research CenterOpen access

In vitro propagation of Gerbera (Gerbera jamesonii Bolus)

Sattar Abdullah Shlahi, Duha Mysire Majeed, Salah Mohammed Hasan

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Abstract

Gerbera plant Gerbera jamesonii is classified according to the flower colors to four strains: white, yellow, pink and purple. Capitulum and scape explants were tested on MS medium in half or full salts strength, supplemented with different combinations of plant growth regulators cytokinins kintin (Kin) and benzel adinine (BA), auxin indolacitic acid (IAA). Results revealed that the capitulum showed better response to shoot formation 64.13% whereas the scape did not show response. Yellow flowers showed higher response in shoot formation 37.5% than other strains. growth regulators combination BA and IAA (3.0 + 0.1) mg/L respectively showed better response for shoot multiplication. Auxin IBA (0.5) mg/ L gave better rooting percentage 60% than other auxins IAA and NAA all concentrations. The acclimatization of the gerbera was 78.59%.

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Gerbera plant Gerbera jamesonii is classified according to the flower colors to four strains: white, yellow, pink and purple. Capitulum and scape explants were tested on MS medium in half or full salts strength, supplemented with different combinations of plant growth regulators cytokinins kintin (Kin) and benzel adinine (BA), auxin indolacitic acid (IAA). Results revealed that the capitulum showed better response to shoot formation 64.13% whereas the scape did not show response. Yellow flowers showed higher response in shoot formation 37.5% than other strains. growth regulators combination BA and IAA (3.0 + 0.1) mg/L respectively showed better response for shoot multiplication. Auxin IBA (0.5) mg/ L gave better rooting percentage 60% than other auxins IAA and NAA all concentrations. The acclimatization of the gerbera was 78.59%.

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Available abstract

Gerbera plant Gerbera jamesonii is classified according to the flower colors to four strains: white, yellow, pink and purple. Capitulum and scape explants were tested on MS medium in half or full salts strength, supplemented with different combinations of plant growth regulators cytokinins kintin (Kin) and benzel adinine (BA), auxin indolacitic acid (IAA). Results revealed that the capitulum showed better response to shoot formation 64.13% whereas the scape did not show response. Yellow flowers showed higher response in shoot formation 37.5% than other strains. growth regulators combination BA and IAA (3.0 + 0.1) mg/L respectively showed better response for shoot multiplication. Auxin IBA (0.5) mg/ L gave better rooting percentage 60% than other auxins IAA and NAA all concentrations. The acclimatization of the gerbera was 78.59%.

Key concepts: Gerbera jamesonii, Gerbera, Auxin, Shoot, Scape, Explant culture, Biology, Botany

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