Vitrification of human ovarian tissue

Cryopreservation of human ovarian tissue has been carried out for more than 18 years.1,2 Successful studies in experimental animals with live births following transplantation of cryostored ovarian tissue in rodents and sheep had been performed earlier.3-6 In the beginning, slow-programmed freezing was utilized. It was also widely applied clinically. For the time being, we can estimate that more than 30 children have been born in this way. ey have been summarized in several articles. e rst live birth aer slowly frozen human ovarian tissue was reported already in 2004,7 followed by many others later on.8-16 Vitrication of human ovarian tissue was the evident next step for improving this methodology.