Measurement of alpha-Amilase Enzymes in Bacillus subtilis Bacteria

The bacterium Bacillus subtilis is able to produce amylase enzymes which are able to hydrolyze various types of starch sources into simple compounds such as maltose, glucose. Amylase converts carbohydrates which are polysaccharides into maltose. Amylase enzyme is a group of enzymes that are needed in the industrial field. The purpose of this study was to determine the level of the amylase enzyme produced by the bacterium Bacillus subtilis. The method used in this research is a quantitative method using an amylase enzyme measurement experiment. The results of this study are from two types of enzyme sources, namely Bacillus subtilis + Starch + NB and Bacillus subtilis + NB. Both samples showed enzyme activity. The conclusion of this study is that Bacillus subtilis + Starch + NB produces 63.186 units of the alpha-amylase enzyme used to produce 1 ppm / 1 micromol maltose per minute. Whereas the Bacillus subtilis + NB sample was 19.474 units of the alpha-amylase enzyme used to produce 1 ppm / 1 micromol maltose per minute.