PERBANDINGAN EFEKTIVITAS ANTIBAKTERI SODIUM HIPOKLORIT 2,5% DAN KOMBINASI SODIUM HIPOKLORIT 2,5% DENGAN CETRIMIDE 0,25% TERHADAP BAKTERI Enterococcus faecalis(IN VITRO)
Fatia Medinah, Rinda Yulianti, Danica Anastasia
Abstract
Fatia Medinah, Rinda Yulianti, Danica Anastasia
Abstract
Background: Sodium hypochlorite (NaOCl) is a common irrigant used for root canal treatment irrigation. Sodium hypochlorite has antibacterial properties but the higher the concentration used, the more toxic it is. HOCl- which is found in sodium hypochlorite works as an antibacterial by damaging cell membranes from bacteria which causes bacterial lysis. Cetrimide is a surfactant that has antibacterial properties and can be combined with sodium hypochlorite. Cetrimide has a antibacterial mechanism similar to sodium hypochlorite in inhibiting bacteria which is damaging bacterial cell membranes. Objective: To determine the effectiveness of antibacterial sodium hypochlorite 2.5% against Enterococcus faecalis compared with combination of sodium hypochlorite 2.5% with cetrimide 0.25%. Methods: This study used a quasi experimental method, consisting of 4 groups (sodium hypochlorite 2.5%, combination of sodium hypochlorite 2.5% with cetrimide 0.25%, cetrimide 0.25% and cetrimide 0.015%). The inhibitory test is carried out by measuring the diameter of the inhibition zone around the well. Data were analyzed by One Way ANOVA test and continued with Post Hoc test. Results: The average inhibitory zone was formed in all groups. Based on the category, the combination of sodium hypochlorite 2.5% with cetrimide 0.25% and sodium hypochlorite 2.5% had very strong antibacterial properties, cetrimide 0.25% and cetrimide 0.015% had strong antibacterial properties. All groups had significant differences except the combination of sodium hypochlorite 2.5% with cetrimide 0.25% which had a inhibition zone of 23.675 mm and sodium hypochlorite 2.5% had 22 mm. Conclusion: The combination of sodium hypochlorite 2.5% with cetrimide 0.25% was more effective than sodium hypochlorite 2.5% as an antibacterial against Enterococcus faecalis.
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Background: Sodium hypochlorite (NaOCl) is a common irrigant used for root canal treatment irrigation. Sodium hypochlorite has antibacterial properties but the higher the concentration used, the more toxic it is. HOCl- which is found in sodium hypochlorite works as an antibacterial by damaging cell membranes from bacteria which causes bacterial lysis. Cetrimide is a surfactant that has antibacterial properties and can be combined with sodium hypochlorite. Cetrimide has a antibacterial mechanism similar to sodium hypochlorite in inhibiting bacteria which is damaging bacterial cell membranes. Objective: To determine the effectiveness of antibacterial sodium hypochlorite 2.5% against Enterococcus faecalis compared with combination of sodium hypochlorite 2.5% with cetrimide 0.25%. Methods: This study used a quasi experimental method, consisting of 4 groups (sodium hypochlorite 2.5%, combination of sodium hypochlorite 2.5% with cetrimide 0.25%, cetrimide 0.25% and cetrimide 0.015%). The inhibitory test is carried out by measuring the diameter of the inhibition zone around the well. Data were analyzed by One Way ANOVA test and continued with Post Hoc test. Results: The average inhibitory zone was formed in all groups. Based on the category, the combination of sodium hypochlorite 2.5% with cetrimide 0.25% and sodium hypochlorite 2.5% had very strong antibacterial properties, cetrimide 0.25% and cetrimide 0.015% had strong antibacterial properties. All groups had significant differences except the combination of sodium hypochlorite 2.5% with cetrimide 0.25% which had a inhibition zone of 23.675 mm and sodium hypochlorite 2.5% had 22 mm. Conclusion: The combination of sodium hypochlorite 2.5% with cetrimide 0.25% was more effective than sodium hypochlorite 2.5% as an antibacterial against Enterococcus faecalis.
Key concepts: Cetrimide, Sodium hypochlorite, Enterococcus faecalis, Chemistry, Disinfectant, Hypochlorite, Sodium, Medicine