Isolation, identification and degradation characteristics of fenpropathrin-degrading strain PSB07-28.
Zhanhong Zhang, Yuanhua Luo, Yong Liu, Songbai Zhang, Deyong Zhang, Xiangwen Luo
Abstract
Zhanhong Zhang, Yuanhua Luo, Yong Liu, Songbai Zhang, Deyong Zhang, Xiangwen Luo
Abstract
A photosynthetic bacterial strain PSB07-28,capable of degrading fenpropathrin,was isolated by enrichment culture. PSB07-28 was identified based on its morphology,physiology and homologic analysis of 16S rDNA sequence(GenBank Accession No. EU004437) . With an evaluation of the degrading characteristics of this strain,an extraction of the degrading-enzyme crude and further testing of the degrading activity,the result shows PSB07-28 is identified as Rhodopseudomonas sp. The optimum conditions of degrading fenpropathrin is 35 ℃,pH7,respectively. This strain could grow in the media supplies with fenpropathrin up to 800 mg/L and degrade 42.49% fenpropathrin in a concentration of 600 mg/L within 15 d. The degrading-enzyme activity results show the degrading-enzyme is endoenzyme.
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A photosynthetic bacterial strain PSB07-28,capable of degrading fenpropathrin,was isolated by enrichment culture. PSB07-28 was identified based on its morphology,physiology and homologic analysis of 16S rDNA sequence(GenBank Accession No. EU004437) . With an evaluation of the degrading characteristics of this strain,an extraction of the degrading-enzyme crude and further testing of the degrading activity,the result shows PSB07-28 is identified as Rhodopseudomonas sp. The optimum conditions of degrading fenpropathrin is 35 ℃,pH7,respectively. This strain could grow in the media supplies with fenpropathrin up to 800 mg/L and degrade 42.49% fenpropathrin in a concentration of 600 mg/L within 15 d. The degrading-enzyme activity results show the degrading-enzyme is endoenzyme.
Key concepts: Strain (injury), 16S ribosomal RNA, GenBank, Enzyme, Isolation (microbiology), Enzyme assay, Food science, Degradation (telecommunications)