2011•Journal of the South China Agricultural UniversityRequires access
The preparation conditions of Spicellum roseum protoplast.
Jiang QianTing, Tai-hui Li, Bin Song
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Abstract
The preparation conditions of Spicellum roseum protoplast including incubation time,enzyme system,enzymolysis time and osmotic stabilizer were studied.The optimal preparation conditions of S.roseum protoplast were 30 h mycelium growth,10 mg/mL cellulase,10 mg/mL snailase and 5 mg/mL lywallzyme,3 h reaction at 30 ℃ and 0.7 mol/L KCl as osmotic stabilizer.