2020•PubMedRequires access
[Antagonistic effect of 12-lipoxygenase inhibitor ML355 on lipopolysaccharide induced inflammatory response in mice].
Xingyu Wang, Wanqi Tang, Tao Chen, Li Luo, Huaping Liang
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Abstract
OBJECTIVE: To explore whether microRNA-21-5p (miR-21-5p) has the effect of anti-apoptosis of human alveolar type II epithelial cells (AT II). METHODS: ). In each group, cell proliferation was detected by cell counting kit-8 (CCK-8) at 0, 12, 24, 36, and 48 hours of cell culture; cell apoptosis was detected by flow cytometry at 24 hours of culture. RESULTS: injury group and miR-21-5p empty virus control group [early apoptosis rate: (14.31±0.12)% vs. (24.50±0.12)%, (23.41±0.13)%; late apoptosis rate: (8.12±0.13)% vs. (9.71±0.11)%, (10.41±0.15)%; overall apoptosis rate: (22.33±0.12)% vs. (34.21±0.10)%, (33.82±0.14)%; all P < 0.05], which further proved that miR-21-5p had anti-apoptotic effects. CONCLUSIONS: miR-21-5p has an anti-apoptotic effect on human AT II.