[Hydrogen plays a protective role in intestinal injury of mice with severe sepsis by regulating the release of heme oxygenase-1 and high mobility group protein B1].

OBJECTIVE: ) inhalation on intestinal injury in severe sepsis mice and its relationship with nuclear factor E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1)/high mobility group protein B1 (HMGB1) pathway. METHODS: for 60 minutes. After modeling, 20 mice randomly in each group were selected to measure 7-day natural survival rates; the remaining mice(15 mice) were killed in 24 hours after modeling, and the bacterial load was determined by collecting the peritoneal lavage fluid (PLF) of each mouse to measure the colony forming unit (CFU); and the intestinal tissue was taken after perfusion, and the intestinal tissue of 3 mice was used to extract protein for HO-1 and HMGB1 levels by using Western blotting. Intestinal tissue of 3 mice was extracted to prepare histomorphology sections for the identification of HO-1. Intestinal tissue of 3 mice was used to detect the expression of HMGB1 by immunofluorescence. RESULTS: group [HO-1 protein expression (HO-1/β-actin): 0.148±0.004 vs. 0.164±0.043, HO-1 positive expression (A value): 0.109±0.020 vs. 0.113±0.025, HMGB1 protein expression (HMGB1/β-actin): 0.109±0.020 vs. 0.113±0.025, HMGB1 positive expression (A value): 39.570±12.660 vs. 42.790±9.680, all P > 0.05]. CONCLUSIONS: .