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2021•Journal of Microbiological MethodsOpen access

The influence of agar brands and micronutrients in the growth optimization of Granulicella sp. (Acidobacteriota)

Ohana Y. A. Costa, Eiko E. Kuramae

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Abstract

Acidobacteriota are highly abundant in soils, however, few cultured representatives are available. The purity of the reagents can influence microbial growth in laboratory conditions and successful isolation. Here we investigated the impact of different agar brands in culture medium and advocate that agar origin should be carefully considered for Acidobacteriota strains growth and microbial isolation.

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Acidobacteriota are highly abundant in soils, however, few cultured representatives are available. The purity of the reagents can influence microbial growth in laboratory conditions and successful isolation. Here we investigated the impact of different agar brands in culture medium and advocate that agar origin should be carefully considered for Acidobacteriota strains growth and microbial isolation.

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Available abstract

Acidobacteriota are highly abundant in soils, however, few cultured representatives are available. The purity of the reagents can influence microbial growth in laboratory conditions and successful isolation. Here we investigated the impact of different agar brands in culture medium and advocate that agar origin should be carefully considered for Acidobacteriota strains growth and microbial isolation.

Key concepts: Agar, Isolation (microbiology), Agar plate, Micronutrient, Biology, Food science, Bacterial growth, Microbiology

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