Phenotypic and molecular characteristics of biofilm and other virulence genes in E. coli and K. pneumoniae isolates from healthy dairy cow, human and environmental sources

Escherichia coli and Klebsiella pneumoniae for ones role in opportunistic infections and pathogenic methodsin veterinary and human medicine, including the formation of biofilms. 33 E.coli and 8 K. pneumoniaeisolates of these micro-organisms isolated from cow’s milk, stools and utensils. Identification were done bybiochemical reaction and confirmed by Polymerase Chain (PCR). These isolates exhibit biofilms formation,as strong, moderate, weak capacities. The expression of the fimA gene. The percentage (84.8%)28/33 ofE.coli was able to produce biofilm while (100%)8/8 in K. pneumoniae The virulence factors of all bacterialisolates were also studied to determine hylA and bla-CTX-M genes in E.coli and, magA and bla-CTX-Mgenes in K. pneumoniae were results presence extended spectrum ?- lactamases (ESBLs). bla-CTX-M genein E. coli and K. pneumoniae (78.7% , 87.5%) It also emerged that not all isolates carry other virulence genesfor this study