PIGMENT ESTIMATION AND ANTI-MICROBIAL PROPERTY OF LEAF EXTRACTS OF DATURA STRAMONIUM L

The present study was carried out on a poisonous plant but with high medicinal values & traditional importance Datura stramonium, for the qualitative and quantitative estimation analysis of primary metabolites & anti-microbial activity .Several methods have been developed for the estimation of these pigments.Plants are the richest source of natural antimicrobial agents.This study aims to conduct an estimation of pigment specifically the chlorophyll content in D. stramonium leaf, using Acetone, Methanol & Ethanol as solvent for extraction, to compare the amount of pigments present in that species, as well as comparison of the antibacterial activity in each solution with Kirby-Bauer standard interpretative method.Fresh leaves of D. stramonium collected from local area of Ranchi, Jharkhand; during January 2020.After the extraction of pigments using the three above mentioned solvents, analysed under UV-VIS spectrophotometry at wavelength 645nm & 663nm.Estimation of chlorophyll content was made using the methods of Arnon (1946).Comparison were made in between young & mature leaf os f Datura stramonium according to efficiency of work in different solvent.Paper Chromatograpy & TLC (Thin layer chromatography) are also used as a part of qualitative estimation of primary and secondary metabolites.Like that for anti bacterial study culture natural agar nutrient prepared by taking 100mg/ml.in 50% DMSO, to grow human pathogenic bacteria, called Staphylococcus aureus & E. coli.