Regeneration in Brassica Juncea L.Crez. (Indian Mustard)

An attempt to regeneration of the Indian mustard (Brassica juncea L.Crez.) in which hypocotyls and cotyledon are used.A quality seed are standardized by surface sterilization using 1 to 2% Nacl solution for 10 min.Sterilized seed are transfer to nutrient medium (MS media ,Cytokinin ), for germination in presence of aseptic condition.We get 5 to 12 cm seedlings in bottle under controlled condition.Select the healthy seedling and separate hypocotyls and cotyledon under laminar air flow (LAR).Again the piece of hypocotyls and cotyledons are transferred to cytokinin known 0.2% medium derived from the MS(Murashige and Skoog) medium for regeneration.And put into controlled condition temperature 18 to 28 degree celcious in suitable light.After 8 day explants proliferated into mass of callus.This indicate the regeneration capacity of Brassica juncea species crop and variation of regeneration varies from hypocotyls to cotyledons.Which further use for genetic study , genetic transformation and crop improvement purpose.