2020•Journal of Cereal ResearchOpen access

Genetic diversity in a set of rice (Oryza sativa L.) genotypes using molecular markers

Yashmeet Kaur, Rupinder Preet Kaur, Renu Khanna, Navjot Singh Sidhu

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Abstract

The genetic diversity among the 94 genotypes was assessed using a set of 48 SSR primers. A total of 129 alleles were detected by 48 primers among 94 rice genotypes with an average of 2.7 alleles per primer. The number of alleles generated by each marker varied from 1 to 4. The PIC values ranged from 0.19 to 0.60 with an average of 0.41. 26 SSR primers revealed PIC values higher than the average. The genetic dissimilarity index ranged from 0.04 to 0.92. The dendrogram generated using Darwin indicated that the 94 genotypes were clustered into three distinct clusters with two sub clusters in each cluster.

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The genetic diversity among the 94 genotypes was assessed using a set of 48 SSR primers. A total of 129 alleles were detected by 48 primers among 94 rice genotypes with an average of 2.7 alleles per primer. The number of alleles generated by each marker varied from 1 to 4. The PIC values ranged from 0.19 to 0.60 with an average of 0.41. 26 SSR primers revealed PIC values higher than the average. The genetic dissimilarity index ranged from 0.04 to 0.92. The dendrogram generated using Darwin indicated that the 94 genotypes were clustered into three distinct clusters with two sub clusters in each cluster.

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Available abstract

The genetic diversity among the 94 genotypes was assessed using a set of 48 SSR primers. A total of 129 alleles were detected by 48 primers among 94 rice genotypes with an average of 2.7 alleles per primer. The number of alleles generated by each marker varied from 1 to 4. The PIC values ranged from 0.19 to 0.60 with an average of 0.41. 26 SSR primers revealed PIC values higher than the average. The genetic dissimilarity index ranged from 0.04 to 0.92. The dendrogram generated using Darwin indicated that the 94 genotypes were clustered into three distinct clusters with two sub clusters in each cluster.

Key concepts: Dendrogram, Genetic diversity, Oryza sativa, Biology, Allele, Genotype, Primer (cosmetics), Genetics

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