2020Meandros Medical And Dental JournalOpen access

Comparison of Two Morphometric Linear Methods to Measure the Alveolar Bone Loss Induced by Experimental Periodontal Disease in Rats

Mehtap Bilgin Çetin, Yasemin Sezgin

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Abstract

Objective: The aim of this study was to compare the direct and indirect linear morphometric measurements for the evaluation of alveolar bone loss after experimentally induced periodontitis in rats. Materials and Methods:Right mandibles of 30 Sprague Dawley rats were collected from a previous study which induced periodontitis using ligature model.In direct measurement group, the distance between cementoenamel junction and alveolar bone crest was evaluated by digital caliper and periodontal probe with a dental loop, whereas indirect group measurement was performed by an image analysis program on digital photographs.Results: The difference between groups was determined using Mann-Whitney U test.Average alveolar bone loss was 1.08±0.22mm in direct measurement group and 1.19±0.33mm inindirect measurement group.The variation between direct and in direct groups was not statistically significant (p>0.05).Conclusion: Based on the present result, it can be concluded that, direct morphometric analysis might be an essential alternative to indirect morphometric evaluations to measure alveolar bone loss after experimentally induced periodontitis in rats.Amaç: Bu çalışmanın amacı, alveoler kemik kaybını değerlendirmek için direkt (alveoler kemik üzerinde dijital bir kumpas ve dental loop ile gerçekleştirilen) ve indirekt (dijital fotoğraflarda özel bir yazılım programı aracılığıyla gerçekleştirilen) doğrusal morfometrik ölçümleri karşılaştırmaktır.Gereç ve Yöntemler: Çalışmamızda kullanılan 30 adet Sprague Dawley sıçan alt çenesi ligatür modeli ile deneysel periodontitis oluşturulmuş önceki bir çalışmadan elde edilmiştir.Mine-sement sınırından alveoler kemik kretine olan mesafe, direkt ölçüm grubunda dijital kumpas ve periodontal sond kullanılarak dental loop ile ölçülürken; indirekt ölçüm grubunda ise özel bir görüntü analiz programı ile dijital fotoğraflar üzerinde gerçekleştirilmiştir.

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Objective: The aim of this study was to compare the direct and indirect linear morphometric measurements for the evaluation of alveolar bone loss after experimentally induced periodontitis in rats. Materials and Methods:Right mandibles of 30 Sprague Dawley rats were collected from a previous study which induced periodontitis using ligature model.In direct measurement group, the distance between cementoenamel junction and alveolar bone crest was evaluated by digital caliper and periodontal probe with a dental loop, whereas indirect group measurement was performed by an image analysis program on digital photographs.Results: The difference between groups was determined using Mann-Whitney U test.Average alveolar bone loss was 1.08±0.22mm in direct measurement group and 1.19±0.33mm inindirect measurement group.The variation between direct and in direct groups was not statistically significant (p>0.05).Conclusion: Based on the present result, it can be concluded that, direct morphometric analysis might be an essential alternative to indirect morphometric evaluations to measure alveolar bone loss after experimentally induced periodontitis in rats.Amaç: Bu çalışmanın amacı, alveoler kemik kaybını değerlendirmek için direkt (alveoler kemik üzerinde dijital bir kumpas ve dental loop ile gerçekleştirilen) ve indirekt (dijital fotoğraflarda özel bir yazılım programı aracılığıyla gerçekleştirilen) doğrusal morfometrik ölçümleri karşılaştırmaktır.Gereç ve Yöntemler: Çalışmamızda kullanılan 30 adet Sprague Dawley sıçan alt çenesi ligatür modeli ile deneysel periodontitis oluşturulmuş önceki bir çalışmadan elde edilmiştir.Mine-sement sınırından alveoler kemik kretine olan mesafe, direkt ölçüm grubunda dijital kumpas ve periodontal sond kullanılarak dental loop ile ölçülürken; indirekt ölçüm grubunda ise özel bir görüntü analiz programı ile dijital fotoğraflar üzerinde gerçekleştirilmiştir.

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Available abstract

Objective: The aim of this study was to compare the direct and indirect linear morphometric measurements for the evaluation of alveolar bone loss after experimentally induced periodontitis in rats. Materials and Methods:Right mandibles of 30 Sprague Dawley rats were collected from a previous study which induced periodontitis using ligature model.In direct measurement group, the distance between cementoenamel junction and alveolar bone crest was evaluated by digital caliper and periodontal probe with a dental loop, whereas indirect group measurement was performed by an image analysis program on digital photographs.Results: The difference between groups was determined using Mann-Whitney U test.Average alveolar bone loss was 1.08±0.22mm in direct measurement group and 1.19±0.33mm inindirect measurement group.The variation between direct and in direct groups was not statistically significant (p>0.05).Conclusion: Based on the present result, it can be concluded that, direct morphometric analysis might be an essential alternative to indirect morphometric evaluations to measure alveolar bone loss after experimentally induced periodontitis in rats.Amaç: Bu çalışmanın amacı, alveoler kemik kaybını değerlendirmek için direkt (alveoler kemik üzerinde dijital bir kumpas ve dental loop ile gerçekleştirilen) ve indirekt (dijital fotoğraflarda özel bir yazılım programı aracılığıyla gerçekleştirilen) doğrusal morfometrik ölçümleri karşılaştırmaktır.Gereç ve Yöntemler: Çalışmamızda kullanılan 30 adet Sprague Dawley sıçan alt çenesi ligatür modeli ile deneysel periodontitis oluşturulmuş önceki bir çalışmadan elde edilmiştir.Mine-sement sınırından alveoler kemik kretine olan mesafe, direkt ölçüm grubunda dijital kumpas ve periodontal sond kullanılarak dental loop ile ölçülürken; indirekt ölçüm grubunda ise özel bir görüntü analiz programı ile dijital fotoğraflar üzerinde gerçekleştirilmiştir.

Key concepts: Dental alveolus, Periodontal disease, Measure (data warehouse), Dentistry, Medicine, Pathology, Orthodontics, Biology

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Comparison of Two Morphometric Linear Methods to Measure the Alveolar Bone Loss Induced by Experimental Periodontal Disease in Rats — Research Paper | ScholarLens