2020•Journal of Clinical MicrobiologyOpen access

Development of a Duplex Real-Time PCR Assay for the Differentiation of Blastomyces dermatitidis and Blastomyces gilchristii and a Retrospective Analysis of Culture and Primary Specimens from Blastomycosis Cases from New York (2005 to 2019)

Mitchell S. Kaplan, YanChun Zhu, Julianne V. Kus, Lisa R. McTaggart, Vishnu P. Chaturvedi, Sudha Chaturvedi

Abstract

Blastomycosis due to Blastomyces dermatitidis and Blastomyces gilchristii is a significant cause of respiratory mycoses in North America with occasional reported outbreaks. We developed a highly sensitive, specific, and reproducible TaqMan duplex real-time PCR assay for the differentiation of B. dermatitidis and B. gilchristii .

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Key concepts: Blastomyces, Blastomyces dermatitidis, Blastomycosis, Biology, Polymerase chain reaction, Microbiology, Pathology, Medicine