HPLC simultaneous determination of schizandrin,imperatorin and osthole in Qirong tablets

Objective:To develop an HPLC method for simultaneous determination of schizandrin,imperatorin and osthole in Qirong tablets.Methods:The analysis was performed on a Diamonsil C18 column(200 mm×4.6 mm,5 μm) with a mobile phase of methanol-water(65∶35).The flow rate was 1.0 mL·min-1 and column temperature was 35 ℃. The detection wavelength was set at 250 nm.Results:The calibration curve was linear within the range of 4-40 μg·mL-1(r=0.9997) for schizandrin,2.46-24.6 μg·mL-1(r=0.9999) for imperatorin,and 16.5-165 μg·mL-1(r=0.9997) for osthole,respectively;The recoveries(n=9) of schizandrin,imperatorin and osthole were 102.3%,101.5%,and 102.0%,respectively. Conclusion:The method is simple,accurate and selective. It can be applied to determine schizandrin,imperatorin and osthole in Qirong tablets.