2020•Cold Spring Harbor ProtocolsRequires access

Detection of Radioactive DNA in Polyacrylamide Gels

Michael R. Green, Joseph Sambrook

Abstract

Bands of radioactive DNA separated by polyacrylamide gel electrophoresis may be detected by autoradiography or phosphorimaging. Analytical polyacrylamide gels containing radioactive DNA are usually fixed and dried before autoradiography. However, if bands of radioactive DNA are to be recovered from the gel, the gel should generally not be fixed or dried.

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Key concepts: Polyacrylamide, Polyacrylamide gel electrophoresis, DNA, Chemistry, Gel electrophoresis, Electrophoresis, Chromatography, Gel electrophoresis of nucleic acids