Effect of supplementation Vitamin E and Selenium in feed containing Lemuru fish oil on blood metabolites and blood profile of broiler chicken

Lemuru fish oil (LFO) can be used as an energy source in poultry diet which contains unsaturated fatty acids. Unsaturated fatty acids are easily oxidized thus require protection. Vitamin E and Selenium (Se) are antioxidants that can protect fatty acids from oxidation. This study aimed to evaluate blood metabolites and blood profiles of broiler fed ration containing LFO with the supplementation of antioxidants in form of different levels of vitamin E and Se. The study used 180 broiler chickens (Lohman MB 202) with 50:50 ratio of male and female reared for 35 days. Randomized factorial design with two factors and three replications were used in this study, as for the first factor is two levels of vitamin E (0 mg kg-1 and 200 mg kg−1) and the second factor is three levels of Se (0 mg kg-1, 0.3 mg kg−1 0.6 mg kg−1). The results showed the use of 200 mg kg−1 of vitamin E showed the increment of the blood hemoglobin, while the use of 0.6 mg kg−1 Se decreased the number of lymphocytes and the combination both vitamin E and Se did not show any significant effect on the performance as well as the blood metabolites and blood profiles. The use of vitamin E 200 mg kg−1 and Se 0.3 mg kg-1 showed a lower H/L ratio compared to the other treatments, but there was no interaction between the two treatments. The supplementation of vitamin E 200 mg kg-1 shows the best results in maintaining the health of broilers based on blood profile results.