[Expression, purification and polyclonal antibody preparation of the Schistosoma japonicum SjGrpE protein].

OBJECTIVE: SjGrpE protein, and to express and purify the recombinant SjGrpE protein and test its immunogenicity. METHODS: BL21, and then IPTG was employed to induce the expression of the target protein, which was purified by nickel ion affinity chromatography. After mice were immunized with the recombinant SjGrpE protein, mouse sera were collected, and the polyclonal antibody against the SjGrpE protein was characterized. RESULTS: gene was approximately 660 bp. The recombinant pET28a-SjGrpE plasmid was successfully generated, and the recombinant SjGrpE protein was obtained following the affinity chromatography, which stimulated mice to secrete high-titer antibodies. CONCLUSIONS: infections.