2020International Journal of Current Microbiology and Applied SciencesOpen access

Invitro Evaluation of Native Rice Specific Isolates of Trichoderma against Rice Sheath Blight caused by Rhizoctonia solani

Konjengbam Sarda Devi, Prabha Devi, Bireswar Sinha, P.H Ranjit Sharma, Nishant Singh, N. Surbala, P. Vignesh, Y. Herojit Singh

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Abstract

Trichoderma is a free living fungi which are highly interactive in root, soil and foliar environments as well. Trichoderma can be used as a biological control agent due to its ability such asmycoparasitism, production of antibiotic and/or hydrolytic enzymes, competition for nutrients, as well as induced plant resistance; production of numerous secondary metabolites inhibitory to the growth of several plant pathogens. In this study, the antagonistic potential of some native rice specific Trichoderma isolates were evaluated against sheath blight disease of rice caused by Rhizoctonia solani. It revealed that the inhibition percentages of R. solani by the native rice specific Trichoderma isolated from various soil samples of Manipur ranges from 62.50% to 87.50% with highest per cent inhibition by WAI-D,T. harzianum (MH257323), and lowest by LAM-B,T. brevicompactum (MH257322) of 87.50% and 62.50% respectively. Bell’s scale study showed class III category by T. brevicompactum (MH257322) and class II showed by T. harzianum (MH257323) against Rhizoctonia solani. Among the native rice specific Trichoderma isolates, WAI-D, T. harzianum (MH257323) is found to be the most effective in reducing the rapid growth of pathogen. Further, all native Trichoderma isolates significantly inhibited the mycelial growth of the pathogen.

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Trichoderma is a free living fungi which are highly interactive in root, soil and foliar environments as well. Trichoderma can be used as a biological control agent due to its ability such asmycoparasitism, production of antibiotic and/or hydrolytic enzymes, competition for nutrients, as well as induced plant resistance; production of numerous secondary metabolites inhibitory to the growth of several plant pathogens. In this study, the antagonistic potential of some native rice specific Trichoderma isolates were evaluated against sheath blight disease of rice caused by Rhizoctonia solani. It revealed that the inhibition percentages of R. solani by the native rice specific Trichoderma isolated from various soil samples of Manipur ranges from 62.50% to 87.50% with highest per cent inhibition by WAI-D,T. harzianum (MH257323), and lowest by LAM-B,T. brevicompactum (MH257322) of 87.50% and 62.50% respectively. Bell’s scale study showed class III category by T. brevicompactum (MH257322) and class II showed by T. harzianum (MH257323) against Rhizoctonia solani. Among the native rice specific Trichoderma isolates, WAI-D, T. harzianum (MH257323) is found to be the most effective in reducing the rapid growth of pathogen. Further, all native Trichoderma isolates significantly inhibited the mycelial growth of the pathogen.

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Available abstract

Trichoderma is a free living fungi which are highly interactive in root, soil and foliar environments as well. Trichoderma can be used as a biological control agent due to its ability such asmycoparasitism, production of antibiotic and/or hydrolytic enzymes, competition for nutrients, as well as induced plant resistance; production of numerous secondary metabolites inhibitory to the growth of several plant pathogens. In this study, the antagonistic potential of some native rice specific Trichoderma isolates were evaluated against sheath blight disease of rice caused by Rhizoctonia solani. It revealed that the inhibition percentages of R. solani by the native rice specific Trichoderma isolated from various soil samples of Manipur ranges from 62.50% to 87.50% with highest per cent inhibition by WAI-D,T. harzianum (MH257323), and lowest by LAM-B,T. brevicompactum (MH257322) of 87.50% and 62.50% respectively. Bell’s scale study showed class III category by T. brevicompactum (MH257322) and class II showed by T. harzianum (MH257323) against Rhizoctonia solani. Among the native rice specific Trichoderma isolates, WAI-D, T. harzianum (MH257323) is found to be the most effective in reducing the rapid growth of pathogen. Further, all native Trichoderma isolates significantly inhibited the mycelial growth of the pathogen.

Key concepts: Rhizoctonia solani, Sheath blight, Rhizoctonia, Biology, Blight, Agronomy, Rice plant, Trichoderma

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