New animal model of induced acute hepatic injury by acetaminophen and Vitamin D3 protective effect

Background: Drug-induced liver injury is of great interest especially drugs that broadly used like acetaminophen. Objective: To assess new model of acetaminophen-induced acute hepatic injury and Vitamin D3 effectiveness. Materials and Methods: Eighteen male rabbits allocated into three groups six rabbits for each. Group 1 receives acetaminophen to induce acute hepatic injury and Group 2 receive single injection of Vitamin D3 before induction of hepatic injury, and group 3 was control. After 24 h from three-spaced injection of acetaminophen sample of blood taken to measure serum level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase, and albumin. Results: Successful model of acetaminophen-induced hepatic injury achieved by significant elevation (P ≤ 0.05) of ALT and AST of Group 1 and 2 compared with control. No significance variation observed (P ≥ 0.05) between treatment Group 1 and 2 in liver function enzymes level. Conclusion: A new easy model of acetaminophen-induced acute hepatic injury effectively obtained. Although no statistical signifi cant Vitamin D3 lower the levels of ALT and AST.