Biochemical studies of different varieties of Indian mustard (Brassica juncea (L.) Czern & Coss) against alternaria blight (Alternaria brassicae (Berk) Sacc.)

Indian mustard (Brassica juncea L. Czern. & Coss.) are the world’s third most important sources of vegetable edible oil. Which is adversely affected by many biotic and abiotic stresses. Among biotic stresses, diseases are the most common factors. Alternaria blight disease caused by the soil-borne necrotrophic fungal pathogen, (Alternaria brassicae (Berk) Sacc.) is a most serious problem in present scenario which damages to all growing part of Mustard including stem, leaves and pods and cruciferous crops worldwide. In present investigation was conducted at the Student’s instructional Farm of N.D. University of Agriculture and Technology Kumarganj Faizabad (U.P.) during rabi season 2016-17.The 10 different hosts were screened in field conditions against Alternaria blight. Disease was appeared on leaves after 45 days of Alternaria brassicae. Giriraj (21.6%), Kranti (22.7%) and RGN-48 (20.5%) were found highly susceptible to Alternaria blight due to higher disease index. Varuna (15.2%) and Pusa Mustard-27 (15.5%) showed the lowest disease index, known as moderately resistant verities. The isozyme of peroxidase and catalase by native -PAGE electrophoresis also showed variability in bands.