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Human hnRNP L Shuttles Between Nucleus and Cytoplasm

So Young Lee, Sung Key Jang, Mie Young Choi

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Abstract

The heterogeneous nuclear ribonucleoprotein L (hnRNP L) is an abundant nuclear protein and one of the major pre-mRNA binding proteins. It is a 68kD protein and contains 558 amino acid residues and four loosely conserved RNP-concensus RNA-binding domains. Recently published data suggest that hnRNP L plays roles on cytoplasmic functions, such as mRNA turnover and regulation of translation. In this study, we investigated the nucleo-cytoplasmic shuttling activity of hnRNP L using transient transfection in conjunction with interspecies heterokaryon assays. Here, we report that hnRNP L; although it is localized primarily in the nucleoplasm, shuttles between nucleus and cytoplasm. The hnRNP L protein is detected mainly in the nucleoplasm by immunofluorescence microscopy, presumably because the return of hnRNP L to the nucleus is very rapid.

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What this paper is about

The heterogeneous nuclear ribonucleoprotein L (hnRNP L) is an abundant nuclear protein and one of the major pre-mRNA binding proteins. It is a 68kD protein and contains 558 amino acid residues and four loosely conserved RNP-concensus RNA-binding domains. Recently published data suggest that hnRNP L plays roles on cytoplasmic functions, such as mRNA turnover and regulation of translation. In this study, we investigated the nucleo-cytoplasmic shuttling activity of hnRNP L using transient transfection in conjunction with interspecies heterokaryon assays. Here, we report that hnRNP L; although it is localized primarily in the nucleoplasm, shuttles between nucleus and cytoplasm. The hnRNP L protein is detected mainly in the nucleoplasm by immunofluorescence microscopy, presumably because the return of hnRNP L to the nucleus is very rapid.

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Available abstract

The heterogeneous nuclear ribonucleoprotein L (hnRNP L) is an abundant nuclear protein and one of the major pre-mRNA binding proteins. It is a 68kD protein and contains 558 amino acid residues and four loosely conserved RNP-concensus RNA-binding domains. Recently published data suggest that hnRNP L plays roles on cytoplasmic functions, such as mRNA turnover and regulation of translation. In this study, we investigated the nucleo-cytoplasmic shuttling activity of hnRNP L using transient transfection in conjunction with interspecies heterokaryon assays. Here, we report that hnRNP L; although it is localized primarily in the nucleoplasm, shuttles between nucleus and cytoplasm. The hnRNP L protein is detected mainly in the nucleoplasm by immunofluorescence microscopy, presumably because the return of hnRNP L to the nucleus is very rapid.

Key concepts: Ribonucleoprotein, Heterogeneous nuclear ribonucleoprotein, Biology, Cytoplasm, Heterogeneous ribonucleoprotein particle, RNA-binding protein, Nuclear protein, Cell nucleus

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