Extraction and characterisation of fungal toxin (Bipolaris sorokiniana) and its effect in different wheat genotypes

Bipolaris sorokiniana is a hemibiotrophic fungus causing spot blotch of wheat. The pathogen produces phytotoxins in culture that induces necrosis and play role in pathogenesis. Different genotypes of wheat were differentiated into susceptible, moderately susceptible and resistant based on the symptom producing ability of that genotype. Our results indicated susceptibility level of wheat to the spot blotch pathogen based on the different genotype and toxin effect of that pathogen. 5 different genotype of wheat were experimented with toxin isolated from the highly virulent isolate of B. sorokiniana, UBS-1.The crude toxin examined under TLC profiling and observed the band morphology. The leaf infiltration bioassay of susceptible, moderately susceptible and resistant varieties of wheat were given the parallel trend of symptom production with toxin treatment.