Evaluation of new generation fungicidal molecules against F. oxysporum f.sp. lycopersici

Tomato fusarium wilt is seen as one of the most important tomato diseases in the field as well as in the world-wide growing greenhouse tomatoes. In presented research, six fungicides; Carbendazim (50% WP), Trifloxystrobin 25% + Tebuconazole 50% WG, Tebuconazole (25% EC), Azoxystrobin (23% EC), Propiconazole (25% EC) and Mancozeb + Carbendazim (75% WP), were evaluated for their efficacy against the disease casual agent Fusarium oxysporum f. sp. lycopersici in vitro and in vivo. Six different concentrations (μg / ml) were used to assess the 0.1, 1, 10, 100, 500 and 1000 ppm active ingredient (a.i) pathogen-inhibiting activities by inhibiting mycelial growth on potato media. Six concentrations of above mentioned fungicides (0.1, 1. 10 and 100 µg / ml) were tested for controlling Fusarium wilt on tomato plants in glasshouse. Measurement of fungal growth inhibition and determination of median appropriate dose (EC50) values (μg / ml); The glasshouse test results showed a varying degree of activity of all fungicides tested in minimizing outbreak of the disease. Out of six fungicides tested against F. oxysporum f. sp. lycopersici at different concentration viz., 0.1ppm, 1ppm, 10 ppm, 100 ppm, 500 ppm and 1000 ppm, Carbendazim @ 1000ppm was found to be the most effective with percent inhibition of 100% followed by Tebuconazole (98.8%), Mancozeb+Carbendazim (97.7%) Propiconazole, and Azoxystrobin (87.7%) while Trifloxystrobin+Tebuconazole was found to be the least effective (85.5%).