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Kinetic Studies of Cultured Human Keratinocytes: A Comparison between 5-Bromodeoxyuridine and Tritiated Thymidine Incorporation

Luisa Benassi, Giovanna Zambruno, Paola Massimi, Fabrizio Fantini, Alberto Gianetti

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Abstract

5-Bromodeoxyuridine (5-BrdU), a pyrimidine analogue of thymidine, is incorporated into the DNA which is actively synthesised during the S-phase of the cell cycle. In the present study, the labelling indices of cultured human keratinocytes, marked by monoclonal antibodies to 5-BrdU and by tritiated thymidine (3H-TdR), were compared. The proportion of cells found to be labelled by the two methods was similar. The immunological method has advantages over 3H-TdR autoradiography, because it does not require radioactive material, and is both faster and less expensive.

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What this paper is about

5-Bromodeoxyuridine (5-BrdU), a pyrimidine analogue of thymidine, is incorporated into the DNA which is actively synthesised during the S-phase of the cell cycle. In the present study, the labelling indices of cultured human keratinocytes, marked by monoclonal antibodies to 5-BrdU and by tritiated thymidine (3H-TdR), were compared. The proportion of cells found to be labelled by the two methods was similar. The immunological method has advantages over 3H-TdR autoradiography, because it does not require radioactive material, and is both faster and less expensive.

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Available abstract

5-Bromodeoxyuridine (5-BrdU), a pyrimidine analogue of thymidine, is incorporated into the DNA which is actively synthesised during the S-phase of the cell cycle. In the present study, the labelling indices of cultured human keratinocytes, marked by monoclonal antibodies to 5-BrdU and by tritiated thymidine (3H-TdR), were compared. The proportion of cells found to be labelled by the two methods was similar. The immunological method has advantages over 3H-TdR autoradiography, because it does not require radioactive material, and is both faster and less expensive.

Key concepts: Thymidine, Bromodeoxyuridine, Labelling, Monoclonal antibody, DNA, Molecular biology, Biology, Chemistry

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Kinetic Studies of Cultured Human Keratinocytes: A Comparison between 5-Bromodeoxyuridine and Tritiated Thymidine Incorporation — Research Paper | ScholarLens