Evaluation of the Correlation between Biofilm Formation and Drug Resistance in Clinical Isolates of Acinetobacter baumannii

Aims: The aim of this study was to determine correlation between biofilm formation and drug resistance in clinical isolates of Acinetobacter baumannii. Study Design: Bacteriological study. Place and Duration of Study: Laboratory of Microbiology of BilecikSeyhEdebali University, in Turkey, between April 2019 and November 2019. Methodology: Antibiotic susceptibility of the strains were determined using Kirby-Bauer disc diffusion method in accordance with the principles of Clinical and Laboratory Standards Institute (CLSI). Biofilm presence in A. baumanniiwas identified by the quantitative method. the isolates were incubated in nutrient agar and was prepared from fresh cultures in tubes containing glucose-Luria-Bertani (LB) medium. The A. baumannii(ATCC 19606) type strain was used for comparisons. Results: In this study was determined the relationship between the biofilm production capacity of the A. baumanniibacteria and its antimicrobial resistance. According to the results obtained from our study, the highest resistance rate (%) was found ceftazidime and piperacillin (95 %) while the highest sensitivity was found colistin (96.6 %) and tigecycline (86.6 %) of the total 60 Acinetobacter baumannii isolates. In addition, the presence of biofilm in the bacteria was defined by quantitative method using microplate. In this study, biofilm was positive in 54 (90 %) isolates and it has been found 51 (85%) of the biofilm positive isolates to be resistant to piperacillin, ceftazidime, cefotaxime and meropenem. Conclusion: As a result, there is a positive relationship between biofilm formation and antibiotic resistance in thesebacteria.