2016•Unpublished venueRequires access
HPLC based estimation and extraction of rutin, quercetin and gallic acid in Moringa oleifera plants grown in Saudi Arabia
M. A. Sharafeldin, Shereen Mohamed Fouad Elkholy, Prawez Alam, Sabry A. Mahfouz
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Abstract
Moringa oleifera L. (Moringaceae) is a valuable medicinal plant with a high nutritional and industrial value. To
analyse the major polyphenolic compounds rutin, quercetin and gallic acid present in the M. oleifera l eaves, HPLC
methods have been used. In the range of 10-30 µg for rutin, quercetin and gallic acid with determinati on coefficient
(R2
) good linearity was observed and linear regression y = 1232.9x + 106.05, y = 133.53x + 202 and y = 61 9.4x +
3442.5 were achieved in 0.98, 0.99 and 1.04 min with the retention time respectively. The LOQs for rutin, quercetin
and gallic acid; 3.4, 10.6 and 4.4 µg/ml and LODs ?4.0 µg/ml for all standards were achieved. The rutin
(555.6±1.79 µg/g), quercetin (37.54±1.07µg/g) and g allic acid (48.5±1.34 µg/g) were recorded in M. ole fera. Rutin
was the most abundant flavonoid analysed in M. oleifera