2009•Journal of Tropical MedicineOpen access
Cloning and sequence analysis of the major allergen β-lactoglobulin in dairy cow (Bos taurus).
Wu Yulan, Zhigang Liu, Xiaowen Chen
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Abstract
Objective Cloning and prokaryotic expression vector construction of β-lactoglobulin(BLG) gene from dairy cow(Bos taurus).Methods The RT-PCR was applied to clone the full-length allergen genes from dairy cow and the sequences were analyzed.The specific primers were designed.The ORF of BLG of dairy cow was subcloned into the expression vector pET28a.Results BLG gene of dairy cow was cloned into expression vector pET28a.The cloned cDNA ORF sequence contained 537 bp and encoded 178 amino acids, with the molecular mass of 19 883, and pI 5.14(GenBank database entry No.EU883600).Sequence analysis showed that this clone shared high identities with BLG from Bos taurus.Conclusion The cow BLG gene was cloned into prokaryotic expression vector.The construct will be used for the expression of the milk allergic protein for further research.