Antifeedant Activity of Ethanolic Leaf Extract of Lantana camara Against Crocidolomia pavonana and Spodoptera litura
Melanie Melanie, Wawan Hermawan, Hikmat Kasmara, Azka Hayyuna Kholifa, Azka Fakhru Rozi
Abstract
Melanie Melanie, Wawan Hermawan, Hikmat Kasmara, Azka Hayyuna Kholifa, Azka Fakhru Rozi
Abstract
Chemical insecticides that have been intensively used to control cabbage caterpillars have become the main causes of pest resistance, high chemical residues in crop products and the decrease of biodiversity of natural enemies in the environment. Secondary metabolites from plant extract provide opportunities to be developed as bioinsecticides. The objective of this research was to evaluate the antifeedant effects of ethanolic leaf extract of L. camara on Crocidolomia pavonana and Spodoptera litura larvae for pre eliminary study. The crude extract was prepared by leaf maceration of L. camara leaf using ethanol, evaporation of the solvent, followed by antifeedant bioassay test. Leaf disc dipping method with choice and no choice tests used to find out the effective minimum concentration on feeding deterrent activity. The mean leaf area consumed were analyzed by using nonparametric statistics of Mann-Whitney U. The results showed that the minimum effective concentration of ethanolic leaf extract that deter the feeding activity of C. pavonana larvae was 2000 ppm (choice test) and 1000 ppm (no choice test) (P 0.05). This performance consider as medium antifeedant category on S.litura larvae at 500 ppm, in concentrations 1000ppm-5000ppm, the extract of L. camara were tended as attractant on S.litura larvae.
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Chemical insecticides that have been intensively used to control cabbage caterpillars have become the main causes of pest resistance, high chemical residues in crop products and the decrease of biodiversity of natural enemies in the environment. Secondary metabolites from plant extract provide opportunities to be developed as bioinsecticides. The objective of this research was to evaluate the antifeedant effects of ethanolic leaf extract of L. camara on Crocidolomia pavonana and Spodoptera litura larvae for pre eliminary study. The crude extract was prepared by leaf maceration of L. camara leaf using ethanol, evaporation of the solvent, followed by antifeedant bioassay test. Leaf disc dipping method with choice and no choice tests used to find out the effective minimum concentration on feeding deterrent activity. The mean leaf area consumed were analyzed by using nonparametric statistics of Mann-Whitney U. The results showed that the minimum effective concentration of ethanolic leaf extract that deter the feeding activity of C. pavonana larvae was 2000 ppm (choice test) and 1000 ppm (no choice test) (P 0.05). This performance consider as medium antifeedant category on S.litura larvae at 500 ppm, in concentrations 1000ppm-5000ppm, the extract of L. camara were tended as attractant on S.litura larvae.
Key concepts: Spodoptera litura, Lantana camara, Maceration (sewage), PEST analysis, Bioassay, Larva, Toxicology, Botany