cDNA Library Construction

Packaging Extract) and a combination of Stratagene Uni-Zap-cDNA Synthesis Kit and Epicentre \nTechnologies MaxPlax&s;" Packaging Extract. MaxPlax"&s; Packaging Extract is a convenient, economical, and simple-to-use reagent to perform in vitro lambda packaging reactions. MaxPlax Packaging \nExtract is supplied as predispensed single-tube reactions that have been optimized for high-efficiency packaging of methylated and unmethylated DNA. The packaging extract routinely yields \npackaging efficiencies of l to 3 x 109 pfu/flg of control DNA. The extract can be used in the \nconstruction of representative eDNA libraries, genomic cloning of highly modified (methylated) \nDNA into -phase or cosmid vectors, and in the rescue of -shuttle vectors from transgenic animal \nsystems. The technical bulletins for the use of these kits are available through Stratagene Cloning \nSystems (LaJolla, CA) and Epicentre Technologies (Madison, WI). With regard to screening of \ntranscribed eDNA, immunoscreening is often employed.