2007•동물실험대체법학회지Requires access
연구논문 : 안점막자극시험의 대체시험법으로서 bovine corneal opacity assay의 확립
노경진, 석승혁, 백민원, Younghee Cheri Lee, 김동재, 나이랑, 박성훈, 이현경, 김배환, 박귀례, 이종권
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Abstract
The objective of this study is establishing of an alternative method using bovine cornea to rabbit eye irritation test. The bovine corneal opacity and permeability assay is checking opacity and permeability of bovine cornea after exposure of chemical irritants. In this study, we measured the opacity of the bovine cornea by quantifying the ability of light to pass through it using opacitometer. In addition, we optimized the basal opacity level before treatment of irritants to bovine corneas. With these results, we established the standard operating procedure of bovine corneal opacity assay.