1980•Proceedings annual meeting Electron Microscopy Society of AmericaRequires access

Adsorption Staining Methd For Ultrathin Frozen Sections

K. T. Tokuyasu

Abstract

The successful routine use of cryoultramicrotomy to examine ultrathin sections by transmission electron microscopy requires the application of suitable staining to delineate the ultrastructure. While negative staining is quite effective for certain purposes1, 2, positive staining is more appropriate for immunocytochemical studies because it does not obscure the immunolabels.

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Key concepts: Staining, Negative stain, Ultrastructure, Transmission electron microscopy, Electron microscope, Chemistry, Pathology, Biophysics