Localization of Cadmium in the Mouse Liver Cells Induced by Acute Cadmium Poisoning

Acute Cadmium-poisoning of a mouse was induced by two intraperitoneal injections of U00 ppm Cadmium chloride solution. 2 hrs after the second injection, the mouse liver was cut into small blocks in the following chilled prefixative. The same volume of 8 % paraformaldehyde and 1/7M veronal acetate buffer solution was mixed and adjusted to pH 7-0, then Oxine was saturated in this fixative. Oxine makes insoluble precipitate with Cadmium(Cd) sensitively(0.1𝛄) Prefixation was proceeded by 2 hrs in ice. The blocks were postfixed with 1 % OsO4, followed by dehydration and embedded in Epon. G-6-Pase(Gomori) and acid phosphatase(Karnovsky) were also examined. Hitachi HU-11E was used for ordinary observations and selected area electron diffraction, and JEOL-IOO-B fitted with nuclear diode Silicon-detector for energy dispersive analysis of X-rays from an Epon embedded section(EDAX), and transmission type scanning electron image system(TSEl).