Determination of glucose content with a concentration within the physiological range by FT-NIR spectroscopy in a trans-reflectance mode

Near-infrared spectroscopy has recently emerged as a powerful apparatus for rapid measurement and mostly does not require a wet chemical analysis. Non-destructive and non-invasive measurements by near-infrared spectroscopy can be realized when operated in all types of trans-reflectance modes. This paper discusses the accuracy of measuring glucose concentrations in aqueous solutions using near-infrared spectroscopy operated in a trans-reflectance mode. In this experiment, glucose with various concentrations was dissolved in water. Forty-seven samples of glucose in aqueous solutions with concentrations between 0 and 800 mg/dL were carefully prepared and divided into two sets, i.e., for calibration and validation. A partial least squares regression analysis on near-infrared spectra was applied. The results revealed that the glucose content in aqueous solutions could be predicted accurately, indicating that the near-infrared prediction is sufficient to determine the glucose content in the physiological range.