Protective effect of carbamylated erythropoietin on neural stem cells in oxygen-glucose deprivation conditions

Objective To observe the protection of carbamylated erythropoietin (CEPO) on neural stem cells (NSCs) impaired by oxygen glucose deprivation (OGD). Methods The NSCs of 14-16 days were isolated, cultured, and passaged. Proliferation, self-renewal ability and multipotency of differentiation of NSCs were examined. CEPO was added to the third passage of NSCs culture exposed to OGD condition for 2h. The apoptosis of NSCs was studied by TUNEL. The proliferation of NSCs was evaluated by MTT method. The level of IFN--γ was detected by ELJSA. Flow cytometry was applied to measure the expression of MHC-Ⅱ in NSCs. Results Under OGD condition, apoptosis of NSCs was 58.97% , and survival rate was 39.46% , and expressions of IFN--γ and MHC- Ⅱ were 78.47 pg/ml and 35.68% , respectively. Supplementation of CEPO displayed significant decrease of apoptosis (30. 15% ) and increase of survival rate (75. 84% ). The level of IFN-7 (15.35 pg/ml) and expression of MHC-H (9.77%) were decreased significantly in CEPO-treated group. Conclusion CEPO can significantly protect NSCs against hypoxia and ischemia, and meanwhile, CEPO can regulate the expression of IFN--γ and MHC- Ⅱ. Key words: Carbamylated erythropoietin;  Neural stem cells;  Oxygen glucose deprivation