The effect of hyperthermia induce on U251 glioma cells apoptosis

Objective To study the effect of different temprature and time points on human glio-ma U251 cells apoptosis induced by hyperthermia. Methods Human U251 glioma cells were cultured in vitro and induced by hyperthermia 43,44,45℃ for 1,2,3,4 hours respectively and then routine culture for 12 hours. The apoptotic cell morphology was observed by Wright-Giemsa staining, the percentage of the cell apoptosis and the alteration of cell cycle were detected by FCM throgh Annexin V-FITC and PI stai-ning. Results The percentage of the cell apoptosis increased with hightening tempreture and prolonging time and the maximum rate of apoptosis was 40.10% at 44 centi-degree for 3 hours. Typical apoptotic bodies or membranosomas appeard. Apoptotic peak and the change of cell cycle were tested by FCM. Con-clusion 3 hours moderate hyperthermia at 43 centi-degree is the effective heat-dosage to induce human glioma U251 cells and the heat-induced apoptotic cells have typical morphology and biochemics character change. Key words: Glioma cell;  Hyperthermia;  Apoptosis;  Flow cytometry