Effects of trophoblast cell-surface antigens 2 gene downregulated by RNA interference on invasion of human prostate carcinoma cells and urokinase-type plasminogen activator protein

Objective To explore the effects and mechanism of trophoblast cell-surface antigens 2 (TROP-2) on invasion of human prostate cancer cells.Methods After human prostate cancer PC-3 cell line was transfected with TROP-2 small interfering RNA (siRNA),the mRNA and protein levels of TROP-2 were determined by using real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blotting respectively.The invasion ability was evaluated by Transwell.The urokinase-type plasminogen activator (uPA) protein of cancer cells was examined by Western blotting.Results The mRNA and protein expression levels of TROP-2 were greatly inhibited in PC-3 cancer cells transfected with TROP-2 siRNA.The results of the Transwell assay showed that the number of cells penetrating the membrane in Con-A,Con-B,and siRNA groups (5,10 and 20 nmoL/L) was 128.16 ± 3.89,127.58 ± 3.56,102.56 ± 3.28,76.38 ± 3.25,and 39.89 ±3.18,respectively (P ＜0.05).The results from Western blotting assay showed that the uPA protein in siRNA groups was reduced significantly.Conclusion TROP-2 siRNA inhibit the invasion of prostate cancer cells through down-regulation of uPA. Key words: Prostate carcinoma;  Trophoblast cell-surface antigens 2 gene;  Invasion;  Urokinase-type plasminogen activator