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2015•Zhonghua mazuixue zazhiRequires access

Effect of dexmedetomidine on spinal p38MAPK expression during remifentanil-induced postoperative hyperalgesia in rats with incisional pain

Xiaoling Huang, Shuiwen Cai, Yi Zou, Lai Wei, Liu Ji-tong

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Abstract

Objective To evaluate the effect of dexmedetomidine on spinal p38 mitogen-activated protein kinase (p38MAPK) expression during remifentanil-induced hyperalgesia in rats with incisional pain. Methods Forty-eight healthy male Sprague-Dawley rats, aged 6 weeks, weighing 220-250 g, were randomly divided into 4 groups (n= 12 each) using a random number table: control group (group C), incision pain group (group IP), incision pain + remifentanil group (group IP+ R), and incision pain + remifentanil + dexmedetomidine group (group IP+ R+ D). After successful establishment of the model of incisionsal pian, remifentanil 1.0 μg/kg was infused for 4 h via the tail vein in group IP+ R; remifentanil 1.0 μg/kg was infused for 4 h via the tail vein, and dexmedetomidine 10 μg/kg was simultaneously infused for 4 h via the jugular vein in group IP+ R+ D; the equal volume of normal saline was infused for 4 h via the tail and jugular veins in C and IP groups.The mechanical paw withdrawal threshold (MWT) was measured at 24 h before operation (T0), and at 4, 6, 24 and 48 h after the end of drug infusion (T1-4). After measurement of MWT at T4, the expression of p38MAPK was determined using immuno-histochemistry. Results Compared with group C, the MWT was significantly decreased at T1-4, and the expression of p38MAPK was up-regulated at T4 in IP and IP+ R groups (P 0.05). Compared with group IP, the MWT was significantly decreased at T1-4, and the expression of p38MAPK was up-regulated at T4 in group P+ R, and the MWT was significantly increased at T1-4, and the expression of p38MAPK was down-regulated at T4 in group IP+ R+ D (P<0.05). Compared with group IP+ R, the MWT was significantly increased at T1-4, and the expression of p38MAPK was down-regulated at T4 in group IP+ R+ D (P<0.05). Conclusion The mechanism by which dexmedetomidine reduces hyperalgesia induced by remifentanil is related to down-regulation of spinal p38MAPK expression in the rats with incisional pain. Key words: Dexmedetomidine; Pain, postoperative; Hyperalgesia; p38 mitogen-activated protein kinases; Spinal cord

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Objective To evaluate the effect of dexmedetomidine on spinal p38 mitogen-activated protein kinase (p38MAPK) expression during remifentanil-induced hyperalgesia in rats with incisional pain. Methods Forty-eight healthy male Sprague-Dawley rats, aged 6 weeks, weighing 220-250 g, were randomly divided into 4 groups (n= 12 each) using a random number table: control group (group C), incision pain group (group IP), incision pain + remifentanil group (group IP+ R), and incision pain + remifentanil + dexmedetomidine group (group IP+ R+ D). After successful establishment of the model of incisionsal pian, remifentanil 1.0 μg/kg was infused for 4 h via the tail vein in group IP+ R; remifentanil 1.0 μg/kg was infused for 4 h via the tail vein, and dexmedetomidine 10 μg/kg was simultaneously infused for 4 h via the jugular vein in group IP+ R+ D; the equal volume of normal saline was infused for 4 h via the tail and jugular veins in C and IP groups.The mechanical paw withdrawal threshold (MWT) was measured at 24 h before operation (T0), and at 4, 6, 24 and 48 h after the end of drug infusion (T1-4). After measurement of MWT at T4, the expression of p38MAPK was determined using immuno-histochemistry. Results Compared with group C, the MWT was significantly decreased at T1-4, and the expression of p38MAPK was up-regulated at T4 in IP and IP+ R groups (P 0.05). Compared with group IP, the MWT was significantly decreased at T1-4, and the expression of p38MAPK was up-regulated at T4 in group P+ R, and the MWT was significantly increased at T1-4, and the expression of p38MAPK was down-regulated at T4 in group IP+ R+ D (P<0.05). Compared with group IP+ R, the MWT was significantly increased at T1-4, and the expression of p38MAPK was down-regulated at T4 in group IP+ R+ D (P<0.05). Conclusion The mechanism by which dexmedetomidine reduces hyperalgesia induced by remifentanil is related to down-regulation of spinal p38MAPK expression in the rats with incisional pain. Key words: Dexmedetomidine; Pain, postoperative; Hyperalgesia; p38 mitogen-activated protein kinases; Spinal cord

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Available abstract

Objective To evaluate the effect of dexmedetomidine on spinal p38 mitogen-activated protein kinase (p38MAPK) expression during remifentanil-induced hyperalgesia in rats with incisional pain. Methods Forty-eight healthy male Sprague-Dawley rats, aged 6 weeks, weighing 220-250 g, were randomly divided into 4 groups (n= 12 each) using a random number table: control group (group C), incision pain group (group IP), incision pain + remifentanil group (group IP+ R), and incision pain + remifentanil + dexmedetomidine group (group IP+ R+ D). After successful establishment of the model of incisionsal pian, remifentanil 1.0 μg/kg was infused for 4 h via the tail vein in group IP+ R; remifentanil 1.0 μg/kg was infused for 4 h via the tail vein, and dexmedetomidine 10 μg/kg was simultaneously infused for 4 h via the jugular vein in group IP+ R+ D; the equal volume of normal saline was infused for 4 h via the tail and jugular veins in C and IP groups.The mechanical paw withdrawal threshold (MWT) was measured at 24 h before operation (T0), and at 4, 6, 24 and 48 h after the end of drug infusion (T1-4). After measurement of MWT at T4, the expression of p38MAPK was determined using immuno-histochemistry. Results Compared with group C, the MWT was significantly decreased at T1-4, and the expression of p38MAPK was up-regulated at T4 in IP and IP+ R groups (P 0.05). Compared with group IP, the MWT was significantly decreased at T1-4, and the expression of p38MAPK was up-regulated at T4 in group P+ R, and the MWT was significantly increased at T1-4, and the expression of p38MAPK was down-regulated at T4 in group IP+ R+ D (P<0.05). Compared with group IP+ R, the MWT was significantly increased at T1-4, and the expression of p38MAPK was down-regulated at T4 in group IP+ R+ D (P<0.05). Conclusion The mechanism by which dexmedetomidine reduces hyperalgesia induced by remifentanil is related to down-regulation of spinal p38MAPK expression in the rats with incisional pain. Key words: Dexmedetomidine; Pain, postoperative; Hyperalgesia; p38 mitogen-activated protein kinases; Spinal cord

Key concepts: Dexmedetomidine, Remifentanil, Medicine, Hyperalgesia, Saline, Anesthesia, Vein, Jugular vein

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Effect of dexmedetomidine on spinal p38MAPK expression during remifentanil-induced postoperative hyperalgesia in rats with incisional pain — Research Paper | ScholarLens