Effect of RNA interference targeting hypoxia inducible factor-1α gene on biological behavior of human pancreatic carcinoma cells

Objective To investigate the effect of hypoxia inducible factor-1ot (HIF-1α) on biological behavior of the pancreatic carcinoma cells,and the clinical significance.Methods The recombinant plasmid pGCsi-shHIF-1α was transfected into Panc-1 cells by LipofectamineTM 2000.The inhibitory effect of HIF-1α was measured at protein level by Western blotting under normoxia and hypoxia.In vitro cell adhesion,wound-healing scrape assay,Transwell invasion assay and cell colony formation assay were performed to assess the effect of HIF-1α on the invasive properties of Panc-I cells.Results The expression of HIF-1 α protein in each group of transfected cells was significantly suppressed under normoxia and hypoxia (0.78 ±0.09 and 1.12 ± 0.12,P ＜ 0.01).The migration of the recombinant plasmid-transfected cells was significantly suppressed as compared with that of cells transfected with empty vector (18.3 ± 3.8 and 19.5 ± 3.5 vs.99.6 ± 8.9 and 106.6 ± 9.8,P ＜ 0.05).Colony formation and MTT assays showed that the cell proliferation of the pGCsi-sh HIF-1α transfected cells was decreased as compared with the control groups (1.89 ± 0.16 and 1.98 ± 0.18 vs.1.04±0.11 and1.13±0.09,P＜0.05).Condusion RNA interference-mediated downregulation of HIF-1α can attenuate the proliferation and migration of Panc-1 cells. Key words: Pancreatic neoplasms ;  RNA interference;  Hypoxia-inducible factor-1 α