Protective effect of all-transretinoic acid on renal ischemia-reperfusion injury in rats

Objective To study the protective effect of total trans retinoic acid (ATRA) on renal injury induced by ischemia-reperfusion in rats. Methods 24 Sprague Dawley (SD) rats were divided into 4 groups of ⑴ Normal control group, ⑵ Sham operation group, ⑶ Model group: the model of renal ischemia-reperfusion was established. ⑷ Retinoic acid treatment Group: ATRA were given by gavage for 7 consecutive days. The rats were killed 24 hours after the model, and the serum and renal tissue were collected. Serum creatinine, tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) levels, kidney biopsy, renal tubular score, renal damage and renal tissue apoptosis were detected. Results Compared with the model group, the serum creatinine level decreased and the creatinine clearance rate increased in ATRA treated group (P<0.05); the damage of renal tubular epithelial cells was alleviated in the pathological slices; and the apoptosis rate of renal tubular epithelial cells was decreased (P<0.05). Serum levels of TNF-α and IFN- γ decreased (P<0.05). Conclusions ATRA can protect the renal ischemia-reperfusion injury through inhibiting inflammatory reaction and apoptosis. Key words: Tretinoin/TU; Reperfusion injury/DT; Kidney diseases/DT