2004Zhongguo jiceng yiyaoRequires access

The examination of apoptosis and expression of proliferating cell nuclear antigen in patients with hepatocellular carcinoma

刘习红, 李慧诚, 杨清绪, 吕汉文

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Abstract

目的 探讨经肝动脉化疗栓塞(TACE)后肝癌细胞中增殖细胞核抗原(PCNA)/凋亡细胞比值与肝细胞肝癌分化程度的关系.方法应用原位末端标记法(TUNEL)及免疫组化双染色技术,对60例肝细胞肝癌石蜡包埋组织进行检测.结果不同分化程度癌组织中细胞凋亡和PCNA的阳性率不同.分化程度高,细胞凋亡阳性率高、PCNA阳性率偏低;分化程度低,细胞凋亡阳性率偏低、PCNA阳性率高.随分化程度的降低,PCNA/凋亡细胞的比值显著增加,两者有相关性.结论PCNA/凋亡细胞的比值可作为评估肝细胞肝癌分化程度的指标。

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目的 探讨经肝动脉化疗栓塞(TACE)后肝癌细胞中增殖细胞核抗原(PCNA)/凋亡细胞比值与肝细胞肝癌分化程度的关系.方法应用原位末端标记法(TUNEL)及免疫组化双染色技术,对60例肝细胞肝癌石蜡包埋组织进行检测.结果不同分化程度癌组织中细胞凋亡和PCNA的阳性率不同.分化程度高,细胞凋亡阳性率高、PCNA阳性率偏低;分化程度低,细胞凋亡阳性率偏低、PCNA阳性率高.随分化程度的降低,PCNA/凋亡细胞的比值显著增加,两者有相关性.结论PCNA/凋亡细胞的比值可作为评估肝细胞肝癌分化程度的指标。

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Available abstract

目的 探讨经肝动脉化疗栓塞(TACE)后肝癌细胞中增殖细胞核抗原(PCNA)/凋亡细胞比值与肝细胞肝癌分化程度的关系.方法应用原位末端标记法(TUNEL)及免疫组化双染色技术,对60例肝细胞肝癌石蜡包埋组织进行检测.结果不同分化程度癌组织中细胞凋亡和PCNA的阳性率不同.分化程度高,细胞凋亡阳性率高、PCNA阳性率偏低;分化程度低,细胞凋亡阳性率偏低、PCNA阳性率高.随分化程度的降低,PCNA/凋亡细胞的比值显著增加,两者有相关性.结论PCNA/凋亡细胞的比值可作为评估肝细胞肝癌分化程度的指标。

Key concepts: Medicine, Hepatocellular carcinoma, Proliferating cell nuclear antigen, Apoptosis, Hepatic carcinoma, Antigen, Cancer research, Carcinoma

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