2003•Unpublished venueRequires access

The effect of bupivacaine on tetrodotoxin-resistant and tetrodotoxin-sensitive sodium channels in rat dorsal root ganglion neurons

魏薇, 张琴, 李泱, 毕好生

Abstract

目的观察布比卡因对大鼠背根神经节神经元钠电流的影响,探讨椎管内麻醉的作用机制.方法以全细胞膜片钳技术记录临床浓度的布比卡因0～1 000 μmol/L对急性分离大鼠背根神经元TTX-S和TTX-R钠电流的影响.钳制电压分别为-100 mv和-90 mv ,刺激电压分别为-10 mv和0 mv.结果临床浓度的布比卡因对TTX -S和TTX-R钠电流均有明显的抑制作用,布比卡因的浓度与其抑制强度呈正相关;布比卡因对TTX-S钠电流抑制的半数有效量IC50为17.069 μmol/L(Hill系数为0.8988) ,而TTX-R钠电流的半数有效量IC50为34.511 μmol/L(Hill系数为0.9003).结论临床浓度的布比卡因对背根神经节神经元TTX-S和TTX-R钠电流具有抑制作用,对TTX-S钠电流的阻滞比对TTX-R钠电流的阻滞强1倍左右,其抑制程度随局麻药浓度的增加而增加.

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Key concepts: Tetrodotoxin, Dorsal root ganglion, Bupivacaine, Dorsum, Sodium channel, Ganglion, Chemistry, Sodium