2008•中国综合临床Requires access
The protective effect of Ginsenoside Rg1 on nerves in the mice model of Parkinson's disease
Jiang Liu, Ran Li, Lei Li, Yuxin Zhang, Zuo‐Feng Zhang
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Abstract
Objective To investigate possible mechanism of Ginsenoside Rg1 on dopaminergic nigral neurons apoptosis of Parkinson's disease (PD) and its protective effect.Methods C57BL/6N mice were administrated with 1-Methy-4-phenyl-1,2,3,6-tetrahydropyri-dine (MPTP) to produce chronic PD model,PD mice were observed in behavioral changes.The expression levels of caspase-3 and TH in ventral midbrain were studied with immunohistochemistry and Western blotting.Apoptotic cell numbers were determined by TdT-mediated dUTP-biotin nick end labeling (TUNEL).Results In group treated with Ginsenoside Rg1,the number of TH-positive neurons in SN was only decreased by 31% as compared with the control group (55%)(P<0.01),the expression of caspase-3 was apparently decreased and major expressed in the cytosol of nigral neurons and TUNEL positive cells in SN decreased (P<0.01).Conclusion The neuroprotective effect of Ginsenoside Rg1 on dopaminergic nigral neurons apoptosis of the mice model of Parkinson's disease induced by MPTP is significant,Decreased expression of Caspase-3 may be the major mechanism of Ginsenoside Rg1 for antiapoptosis.
Key words:
Parkinson's disease; Ginsenoside ng1; Apoptosis; Dopamine