Effect of target silencing FGF8b gene on proliferation and apoptosis of ovarian cancer SKOV-3 cells

Objective To investigate the effect of targeted silencing FGF8b gene on the proliferation and apoptosis of ovarian cancer SKOV-3 cells and its possible molecular mechanism. Methods The design and synthesis of small interfering RNA targeting FGF8b (siRNA) , was transfected into ovarian cancer cell SKOV-3, using four methyl thiazolyl tetrazolium (MTT) method to detect cell proliferation and apoptosis of TUNEL kit cells. The expression of Bcl-2 and Bax protein by Western blot assay in cells. Results FGF8b siRNA could inhibit the expression of FGF8b protein in ovarian cancer SKOV-3 cells. The experimental group showed a high proportion of TUNEL positive cells (22.33±2.517) (P<0.01) . The down-regulation of FGF8b protein significantly inhibited the proliferation of ovarian cancer SKOV-3 cells, reduced the expression of Bcl-2 (0.586±0.025) (P<0.01) , and increased the expression of Bax (0.334±0.019) (P<0.01) . Conclusion Down regulation of FGF8b expression can inhibit the proliferation of ovarian cancer SKOV-3 cells and induce cell apoptosis, which may be related to the expression changes of Bcl-2 and Bax protein, suggesting that FGF8b plays an important role in the development of ovarian cancer. Key words: FGF8b; Ovarian cancer; RNA interference; Cell proliferation; Cell apoptosis