The expression and significance of IL-6 receptor and IL-8 receptor in a rat model of ulcerative colitis

Objective To discuss the expression and significance of IL-6 receptor and IL-8 receptor in a rat model of ulcerative colitis. Methods Thirty-six SD rats were randomly divided into 3 groups, twelve rats in each group. group 1:normal; group 2:blank 1; group 3:blank 2.The rat model of ulcerative colitis was brought about by an enema of 100 mg/kg of TNBS and 5% ethanol. The blank 1 rat was sacrificed 1 week later. The other rats were sacrificed 2 weeks later. The expression of IL-6R and IL-8R were detected with the technique of ELISA and Real-Time PCR. Results The mRNA and protein expression of IL-6R were (3.35±0.12)pg/ml,(111.35±25.09)pg/ml and (3.56±0.09)pg/ml,(160.11±15.54)pg/ml in blank 1 and blank 2.While the mRNA and protein expression of IL-8R were(3.50±0.19)pg/ml,(160.11±15.54)pg/ml and(3.68±0.16)pg/ml,(185.38±15.42)pg/ml respectively. Compared to normal group, there was a significant difference in the results of CINC-1 and CXCR2 (P<0.01). Conclusions The mRNA and protein expression of IL-6R and IL-8R is on the increment in active UC.There is relation between UC and the expression of IL-6R and IL-8R. Key words: Ulcerative colitis;  Interleukin-6 receptor;  Interleukin-8 receptor.