The expression and role of Caspase3, Caspase12 and CHOP in pituitary tissues of rats with acute necrotizing pancreatitis

Objective To observe the pathological changes of pituitary tissue in rats with acute necrotizing pancreatitis and to explore the mechanism of pituitary tissue injury in rats. Methods 24 SD rats were randomly divided into normal group (N group, n=8) , sham operation group (SO group, n=8) , and acute necrotizing pancreatitis group (ANP group, n=8) . ANP model was established by retrograde injection of 5% sodium taurocholate into the biliopancretic duct. The serum levels of amylase (AMY) and lipase (LIP) were detected by automatic biochemical. The serum levels of growth hormone (GH) , adrenocorticotropic hormone (ACTH) , thyroid stimulating hormone (TSH) and follicle-stimulating hormone (FSH) were measured by radioimmunoassay. The pathological changes of pancreatic tissue and pituitary tissue were observed by the light microscope. The expression of Casepase3, Caspase12 and CHOP in pituitary tissue were determined by immunohistochemical method. Results Compared to SO group, the serum levels of AMY (8679.16±307.60) U/L and LIP (9376.83±380.92) U/L were significantly higher in ANP group (P<0.05) . The serum levels of ACTH (0.92±0.41) pg/ml, TSH (0.14±0.06) pg/ml, and FSH (2.01±0.38) pg/ml were significantly lower in ANP group (P<0.05) . The expression of Caspse 3 (65.66±7.58) , Caspase12 (70.66±4.76) and CHOP (143.16±19.05) in pituitary tissue were significantly increased in ANP group (P<0.05) . The pancreatic injury was more severe in ANP group under light microscope (P<0.05) . The degree of hyperemia of pituitary tissue of ANP group was aggravated. Conclusion Pathological changes occur in rat pituitary tissues and endoplasmic reticulum stress injury plays a role in pituitary injury during ANP. Key words: Acute necrosis pancreatitis; Pituitary; Apoptosis; Caspase3; Caspase12; CHOP