2018•Chinese Journal of NeuromedicineRequires access
Effect of bone marrow mesenchymal stem cells transplantation on recovery of neurological functions and Nogo-A expression in cerebral ishchemia rats at recovery stage
Zhenjun Li, Wenchao Liu, Chuanzhi Duan, Xin Zhang, Xifeng Li, Wenxian Zeng, Jianbo Zhang
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Abstract
Objective
To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on recovery of neurological functions and Nogo-A expression in cerebral ischemia rats at recovery stage.
Methods
BMSCs were isolated and cultured by whole bone marrow adherence method. Thirty-six SD rats were randomized into sham-operated group, ischemia group and BMSCs transplantation group (n=12). The middle cerebral artery occlusion (MCAO) models in the ischemia group and BMSCs transplantation group were established with Zea Longa line embolism. After 21 d of MCAO, one mL of BMSCs (3×106) were transplanted into rats of the BMSCs transplantation group, and same amount of phosphate buffer was given to the rats of the sham-operated group and ischemia group. After 14 and 28 d of treatment, neurological functions of the rats were evaluated by modified neurological severity scale (mNSS); the brain infarct sizes were tested by TTC staining; the pathological alterations were tested by HE staining, and the Nogo-A expression was determined by immunofluorescence.
Results
After 14 and 28 d of treatment, as compared with the ischemia group, BMSCs group had significantly lower mNSS scores (14 d: 7.50±0.55 vs. 6.17±0.75; 28 d: 7.33±0.52 vs. 5.67±0.82), statistically smaller brain infarct sizes (14 d: 31.38%±1.02% vs. 26.32%±1.19%; 28 d: 27.71%±0.55% vs. 21.68%±1.09%), and significantly lower Nogo-A expression (14 d: 39.33%±2.08% vs. 33.67%±2.52%; 28 d: 30.33%±0.58% vs. 25.67%±4.39%, P<0.05). What’s more, rats in the BMSCs group had milder cell damage and decreased scar tissues as compared with those in the ischemia group.
Conclusion
BMSCs transplantation can improve the neurological function of cerebral ischemia rats at recovery stage, and it may work via regulation of Nogo-A expression.
Key words:
Cerebral ischemia; Recovery stage; Bone marrow mesenchymal stem cell; Nogo-A; Cell transplantation