Study on the expansion of umbilical cord blood mononuclear cells supported by human umbilical cord blood mesenchymal stem cells

Objective To explore the effects on umbilical cord blood mesenchymal stem cells (UCB-MSCs) combined with exogenous cytokines in supporting the expansion of umbilical cord blood mononuclear cells(UCB MNCs). Methods Mesenchymal stem cells (MSCs) were isolated from umbilical cord blood, and then were detected the cellular surface antigens. The UCB-MNCs were euhured in a serum-free system for 18 days. On day 0, 7, 10, 14 and 18, total mononuclear cells、CD34 cells、CD133 cells、cellular cycle and isolated and cultured from umbilical cord blood were negative for CD34 and CD133, but positive for CD29, The expansion combined with UCB-MSCs and exogenous cytokines had better effect than another groups. The above parameters reached the peak values on day 10. The culture system could maintain at least 18 efficiently support the expansion of UCB-MNCs. Key words: Umbilical cord blood;  Cytokines;  Mesenehymal stem cells;  Cell culture